To quantify mitochondrial accumulation at the junctions, we utilized multiple immunolabeling, correlated confocal laser scanning , and a semi-automated unbiased technique (Figures 2L-2S).
We discovered that, at every investigated age, TOM20 immunofluorescence intensity in the cell our bodies and proximal tufts of DCX+ neurons was substantially larger at websites of microglial contact compared with adjacent areas . These results confirmed that neuronal mitochondria are strongly enriched at somatic contacts. These junctions possess a unique ultrastructural and molecular composition …